Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Simultaneous Quantification of Paracetamol and Meloxicam in Tablets by High Performance Liquid Chromatography

Madhusudhanareddy Induri¹ , Bhagavan R Mantripragada², Rajendra P Yejella³, Pavankumar R Kunda², Meechel Arugula⁴, Rajkumar Boddu¹

¹Department of Pharmaceutical Chemistry, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad, Andhra Pradesh 500014; ²Department of Pharmaceutical Chemistry, C.M. College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad, Andhra Pradesh 500014; ³Department of Pharmaceutical Chemistry, College of Pharmaceutical Sciences, Andhra University, Visakhapatnam, Andhra Pradesh 530003; ⁴Department of Pharmaceutical Analysis, St. Ann's College of Pharmacy, Cantonment, Vizianagaram, Andhra Pradesh 530003, India.

For correspondence:- Madhusudhanareddy Induri Email: msreddyinduri@yahoo.com Tel:+918125863579

Received: 5 December 2010 Accepted: 26 June 2011 Published: 20 August 2011

Citation: Induri M, Mantripragada BR, Yejella RP, Kunda PR, Arugula M, Boddu R. Simultaneous Quantification of Paracetamol and Meloxicam in Tablets by High Performance Liquid Chromatography. Trop J Pharm Res 2011; 10(4):475-481 doi: 10.4314/tjpr.v10i4.13

© 2011 The authors.
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Abstract

Purpose: To develop and validate a simple, rapid and inexpensive RP-HPLC method for the simultaneous estimation of paracetamol and meloxicam in tablets.

Methods: For the analysis of the drugs, chromatographic analysis was performed on XTerra symmetry C₁₈ column (100 × 4.6 mm, 5 µ particle size) with mobile phase consisting of methanol and phosphate buffer (pH 9.2) in the ratio of 50:50 v/v, at a flow rate of 0.8 mL/min and eluents monitored at 244 nm. The method was validated for linearity, accuracy, precision, robustness and application for assay as per International Conference on Harmonization (ICH) guidelines.

Results: The retention times of paracetamol and meloxicam were 2.467 and 4.971 min, respectively. The calibration curves of peak area versus concentration, which was linear from 5 - 60 µg/mL for paracetamol and 1 - 12 µg/mL for meloxicam, had regression coefficient (r²) greater than 0.999. The method had the requisite accuracy, precision, and robustness for simultaneous determination of paracetamol and meloxicam in tablets.

Conclusion: The proposed method is simple, low-cost, accurate, precise and can be successfully employed in routine quality control for the simultaneous analysis of paracetamol and meloxicam in tablets.

Keywords: Paracetamol, Meloxicam, RP-HPLC, Simultaneous analysis, Tablets